Characterization and statistical optimization of biosurfactant production using Bacillus subtilis isolated from automotive oil-contaminated soil in Yola, Nigeria

The need for cost effective and ecofriendly means of producing compounds environmental, industrial, other biotechnological applications cannot be overemphasized. Biosurfactant from microbial sources have been identified to the potential substitute chemical types. This has led continuous bioprospecting biosurfactant-producing microorganisms optimization processes high yield biosurfactant production. Despite widespread, levels oil-induced soil pollution in Yola, Nigeria its environs, there is dearth information on application indigenous bacteria In this study, a Bacillus subtilis B3 strain was isolated an automotive oil-contaminated further used production, characterization, activity. partially purified had structural elucidation lipopeptide exhibited surface tension reduction distilled water 72 29.30 mN/m. It also forms stable emulsions between pH 6-8 temperature 60-80 °C, critical micelle concentration 0.65 %. dry weight 0.25 g/L representing 1.6-fold increase comparison with one obtained conventional medium 0.16 yield. finding suggests that bacterial study meet demand bioremediation industrial exploitations.